Coding

Part:BBa_K209407:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-09-30)

AarI C-D part, SOS1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1790
    Illegal EcoRI site found at 2634
    Illegal SpeI site found at 831
    Illegal PstI site found at 413
    Illegal PstI site found at 3166
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1790
    Illegal EcoRI site found at 2634
    Illegal NheI site found at 1447
    Illegal SpeI site found at 831
    Illegal PstI site found at 413
    Illegal PstI site found at 3166
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1790
    Illegal EcoRI site found at 2634
    Illegal BglII site found at 2379
    Illegal BglII site found at 3391
    Illegal BamHI site found at 3200
    Illegal XhoI site found at 112
    Illegal XhoI site found at 896
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1790
    Illegal EcoRI site found at 2634
    Illegal SpeI site found at 831
    Illegal PstI site found at 413
    Illegal PstI site found at 3166
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1790
    Illegal EcoRI site found at 2634
    Illegal SpeI site found at 831
    Illegal PstI site found at 413
    Illegal PstI site found at 3166
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 130
    Illegal SapI.rc site found at 571


Design Notes

Cloned into pDONR221.

Source

References